PUBLICATIONS
2020
Tejada-Jimenez, M., Llamas, A., Galván, A., Fernández, E. Role of nitrate reductase in NO production in photosynthetic eukaryotes (2019) Plants, 8 (3), art. no. 56. DOI: 10.3390/plants8030056
Calatrava, V., Hom, E.F.Y., Llamas, Á., Fernández, E., Galván, A. Nitrogen scavenging from amino acids and peptides in the model alga Chlamydomonas reinhardtii. The role of extracellular L-amino oxidase (2019) Algal Research, 38, art. no. 101395. DOI: 10.1016/j.algal.2018.101395
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Gil-Díez P, Tejada-Jiménez M*, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero* M. MtMOT1.2 is responsible for molybdate supply to Medicago truncatula nodules (2019) Plant Cell Environ. Jan;42(1):310-320. doi: 10.1111/pce.13388
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Tejada-Jimenez, M., Chamizo-Ampudia, A., Calatrava, V., Galván, A., Fernández, E., Llamas, A. From the eukaryotic molybdenum cofactor biosynthesis to the moonlighting enzyme MARC (2018) Molecules, 23 (12), art. no. 3287. DOI: 10.3390/molecules23123287
Ariz, I., Boeckstaens, M., Gouveia, C., Martins, A.P., Sanz-Luque, E., Fernández, E., Soveral, G., Von Wirén, N., Marini, A.M., Aparicio-Tejo, P.M., Cruz, C. Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily (2018) Science Advances, 4 (9), art. no. eaar3599. DOI: 10.1126/sciadv.aar3599
Calatrava, V., Hom, E.F.Y., Llamas, Á., Fernández, E., Galván, A. OK, thanks! A new mutualism between Chlamydomonas and methylobacteria facilitates growth on amino acids and peptides (2018) FEMS Microbiology Letters, 365 (7), art. no. fny021. DOI: 10.1093/femsle/fny021
González-Ballester, D., Sanz-Luque, E., Galván, A., Fernández, E., de Montaigu, A. Arginine is a component of the ammonium-CYG56 signalling cascade that represses genes of the nitrogen assimilation pathway in Chlamydomonas reinhardtii (2018) PLoS ONE, 13 (4), art. no. e0196167. DOI: 10.1371/journal.pone.0196167
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Tejada-Jiménez M*, Gil-Díez P, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M*. Medicago truncatula Molybdate Transporter type 1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency (2017) New Phytol. 2017 Dec;216(4):1223-1235. doi: 10.1111/nph.14739
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Llamas, A., Chamizo-Ampudia, A., Tejada-Jimenez, M., Galván, A., Fernández, E. The molybdenum cofactor enzyme mARC: Moonlighting or promiscuous enzyme? (2017) BioFactors, 43 (4), pp. 486-494. DOI: 10.1002/biof.1362
Plouviez, M., Wheeler, D., Shilton, A., Packer, M.A., McLenachan, P.A., Sanz-Luque, E., Ocaña-Calahorro, F., Fernández, E., Guieysse, B. The biosynthesis of nitrous oxide in the green alga Chlamydomonas reinhardtii (2017) Plant Journal, 91 (1), pp. 45-56. DOI: 10.1111/tpj.13544
González-Ballester, D., Jurado-Oller, J.L., Galván, A., Fernández, E., Dubini, A. H2 production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article "on the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions," by Alberto Scoma and Szilvia Z. Tóth (2017) Biotechnology for Biofuels, 10 (1), art. no. 117. DOI: 10.1186/s13068-017-0801-5
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Abreu I, Saéz Á, Castro-Rodríguez R, Escudero V, Rodríguez-Haas B, Senovilla M, Larue C, Grolimund D, Tejada-Jiménez M, Imperial J, González-Guerrero M. Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells (2017) Plant Cell Environ. 2017 Nov;40(11):2706-2719. doi: 10.1111/pce.13035
Calatrava, V., Chamizo-Ampudia, A., Sanz-Luque, E., Ocaña-Calahorro, F., Llamas, A., Fernández, E., Galván, A. How Chlamydomonas handles nitrate and the nitric oxide cycle (2017) Journal of Experimental Botany, 68 (10), pp. 2593-2602. DOI: 10.1093/jxb/erw507
Chamizo-Ampudia, A., Galván, A., Fernández, E., Llamas, A. Study of different variants of Mo enzyme crARC and the interaction with its partners crCytb5-R and crCytb5-1 (2017) International Journal of Molecular Sciences, 18 (3), art. no. 670. DOI: 10.3390/ijms18030670
Chamizo-Ampudia, A., Sanz-Luque, E., Llamas, A., Galván, A., Fernández, E. Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis (2017) Trends in Plant Science, 22 (2), pp. 163-174. DOI: 10.1016/j.tplants.2016.12.001
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Chamizo-Ampudia, A., Sanz-Luque, E., Llamas, Á., Ocaña-Calahorro, F., Mariscal, V., Carreras, A., Barroso, J.B., Galván, A., Fernández, E. A dual system formed by the ARC and NR molybdoenzymes mediates nitrite-dependent NO production in Chlamydomonas (2016) Plant Cell and Environment, 39 (10), pp. 2097-2107. DOI: 10.1111/pce.12739
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González-Guerrero M, Escudero V, Saéz Á, Tejada-Jiménez M. Transition Metal Transport in Plants and Associated Endosymbionts: Arbuscular Mycorrhizal Fungi and Rhizobia (2106) Front Plant Sci. 7:1088. doi: 10.3389/fpls.2016.01088
Sanz-Luque, E., Ocaña-Calahorro, F., Galván, A., Fernández, E., de Montaigu, A. Characterization of a Mutant Deficient for Ammonium and Nitric Oxide Signalling in the Model System Chlamydomonas reinhardtii (2016) PLoS ONE, 11 (5), art. no. e0155128. DOI: 10.1371/journal.pone.0155128
Higuera, J.J., Calatrava, V., González, Z., Mariscal, V., Siverio, J.M., Fernández, E., Galván, A. NRT2.4 and NRT2.5 are two half-size transporters from the Chlamydomonas NRT2 family (2016) Agronomy, 6 (1), art. no. 20. DOI: 10.3390/agronomy6010020
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Sanz-Luque, E., Chamizo-Ampudia, A., Llamas, A., Galván, A., Fernández, E. Understanding nitrate assimilation and its regulation in microalgae (2015) Frontiers in Plant Science, 6, art. no. 899. DOI: 10.3389/fpls.2015.00899
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Tejada-Jiménez M, Castro-Rodríguez R, Kryvoruchko I, Lucas MM, Udvardi M, Imperial J, González-Guerrero M. Medicago truncatula natural resistance-associated macrophage Protein1 is required for iron uptake by rhizobia-infected nodule cells (2015) Plant Physiol. 168(1):258-72. doi: 10.1104/pp.114.254672
Jurado-Oller, J.L., Dubini, A., Galván, A., Fernández, E., González-Ballester, D. Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures (2015) Biotechnology for Biofuels, 8 (1), art. no. 149. DOI: 10.1186/s13068-015-0341-9
González-Ballester, D., Jurado-Oller, J.L., Fernández, E. Relevance of nutrient media composition for hydrogen production in Chlamydomonas (2015) Photosynthesis Research, 125 (3), pp. 395-406. DOI: 10.1007/s11120-015-0152-7
Sanz-Luque, E., Ocaña-Calahorro, F., De Montaigu, A., Chamizo-Ampudia, A., Llamas, A., Galván, A., Fernández, E. THB1, a truncated hemoglobin, modulates nitric oxide levels and nitrate reductase activity (2015) Plant Journal, 81 (3), pp. 467-479. DOI: 10.1111/tpj.12744
Sanz-Luque, E., Ocañna-Calahorro, F., Galván, A., Fernández, E. THB1 regulates nitrate reductase activity and THB1 and THB2 transcription differentially respond to NO and the nitrate/ammonium balance in Chlamydomonas (2015) Plant Signaling and Behavior, 10 (8), 4 p. DOI: 10.1080/15592324.2015.1042638
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Higuera, J.J., Fernández, E., Galván, A. Chlamydomonas NZF1, a tandem-repeated zinc finger factor involved in nitrate signalling by controlling the regulatory gene NIT2 (2014) Plant, Cell and Environment, 37 (9), pp. 2139-2150. DOI: 10.1111/pce.12305
Remacle, C., Eppe, G., Coosemans, N., Fernández, E., Vigeolas, H. Combined intracellular nitrate and NIT2 effects on storage carbohydrate metabolism in Chlamydomonas (2014) Journal of Experimental Botany, 65 (1), pp. 23-33. DOI: 10.1093/jxb/ert339
Léran, S., Varala, K., Boyer, J.-C., Chiurazzi, M., Crawford, N., Daniel-Vedele, F., David, L., Dickstein, R., Fernández, E., Forde, B., Gassmann, W., Geiger, D., Gojon, A., Gong, J.-M., Halkier, B.A., Harris, J.M., Hedrich, R., Limami, A.M., Rentsch, D., Seo, M., Tsay, Y.-F., Zhang, M., Coruzzi, G., Lacombe, B. A unified nomenclature of nitrate transporter 1/peptide transporter family members in plants (2014) Trends in Plant Science, 19 (1), pp. 5-9. DOI: 10.1016/j.tplants.2013.08.008
Tejada-Jiménez, M., Chamizo-Ampudia, A., Galván, A., Fernández, E., Llamas, Á. Molybdenum metabolism in plants (2013) Metallomics, 5 (9), pp. 1191-1203. DOI: 10.1039/c3mt00078h
Sanz-Luque, E., Ocaña-Calahorro, F., Llamas, A., Galván, A., Fernández, E. Nitric oxide controls nitrate and ammonium assimilation in Chlamydomonas reinhardtii (2013) Journal of Experimental Botany, 64 (11), pp. 3373-3383. DOI: 10.1093/jxb/ert175
Chamizo-Ampudia, A., Galván, A., Fernández, E., Llamas, A. Characterization of Chlamydomonas 102 and 104 Mutants Reveals Intermolecular Complementation in the Molybdenum Cofactor Protein CNX1E (2013) Protist, 164 (1), pp. 116-128. DOI: 10.1016/j.protis.2012.04.003
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Vila, M., Galván, A., Fernández, E., León, R. Ketocarotenoid biosynthesis in transgenic microalgae expressing a foreign β-C-4-carotene oxygenase gene (2012) Methods in Molecular Biology, 892, pp. 283-295. DOI: 10.1007/978-1-61779-879-5_17
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Chamizo-Ampudia, A., Galván, A., Fernández, E., Llamas, A. The Chlamydomonas reinhardtii Molybdenum Cofactor Enzyme crARC has a Zn-Dependent Activity and Protein Partners Similar to Those of its Human Homologue (2011) Eukaryotic Cell, 10 (10), pp. 1270-1282. DOI: 10.1128/EC.05096-11
González-Ballester, D., Pootakham, W., Mus, F., Yang, W., Catalanotti, C., Magneschi, L., De Montaigu, A., Higuera, J.J., Prior, M., Galván, A., Fernández, E., Grossman, A.R. Reverse genetics in Chlamydomonas: A platform for isolating insertional mutants (2011) Plant Methods, 7 (1), art. no. 24. DOI: 10.1186/1746-4811-7-24
Llamas, Á., Tejada-Jiménez, M., Fernández, E., Galván, A. Molybdenum metabolism in the alga Chlamydomonas stands at the crossroad of those in Arabidopsis and humans (2011) Metallomics, 3 (6), pp. 578-590. DOI: 10.1039/c1mt00032b
Tejada-Jiménez, M., Galván, A., Fernández, E. Algae and humans share a molybdate transporter (2011) Proceedings of the National Academy of Sciences of the United States of America, 108 (16), pp. 6420-6425. DOI: 10.1073/pnas.1100700108
De Montaigu, A., Sanz-Luque, E., Macias, M.I., Galván, A., Fernández, E. Transcriptional regulation of CDP1 and CYG56 is required for proper NH 4+ sensing in Chlamydomonas (2011) Journal of Experimental Botany, 62 (4), pp. 1425-1437. DOI: 10.1093/jxb/erq384
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Ermilova, E.V., Zalutskaya, Z.M., Nikitin, M.M., Lapina, T.V., Fernández, E. Regulation by light of ammonium transport systems in Chlamydomonas reinhardtii (2010) Plant, Cell and Environment, 33 (6), pp. 1049-1056. DOI: 10.1111/j.1365-3040.2010.02126.x
de Montaigu, A., Sanz-Luque, E., Galván, A.G., Fernández, E. A soluble guanylate cyclase mediates negative signaling by ammonium on expression of nitrate reductase in Chlamydomonas (2010) Plant Cell, 22 (5), pp. 1532-1548. DOI: 10.1105/tpc.108.062380
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Fernández, E., Llamas, A., Galván, A. Nitrogen Assimilation and its Regulation (2009) The Chlamydomonas Sourcebook 3-Vol set, 2, pp. 69-113. DOI: 10.1016/B978-0-12-370873-1.00011-3
Tejada-Jiménez, M., Galván, A., Fernández, E., Llamas, A. Homeostasis of the micronutrients Ni, Mo and Cl with specific biochemical functions (2009) Current Opinion in Plant Biology, 12 (3), pp. 358-363. DOI: 10.1016/j.pbi.2009.04.012
Castaings, L., Camargo, A., Pocholle, D., Gaudon, V., Texier, Y., Boutet-Mercey, S., Taconnat, L., Renou, J.-P., Daniel-Vedele, F., Fernández, E., Meyer, C., Krapp, A. The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis (2009) Plant Journal, 57 (3), pp. 426-435. DOI: 10.1111/j.1365-313X.2008.03695.x
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Fernández, E., Galván, A. Nitrate assimilation in Chlamydomonas (2008) Eukaryotic Cell, 7 (4), pp. 555-559. DOI: 10.1128/EC.00431-07
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Tejada-Jiménez, M., Llamas, Á., Sanz-Luque, E., Galván, A., Fernández, E. A high-affinity molybdate transporter in eukaryotes (2007) Proceedings of the National Academy of Sciences of the United States of America, 104 (50), pp. 20126-20130. DOI: 10.1073/pnas.0704646104
Léon, R., Galván, A., Fernández, E. Advances in Experimental Medicine and Biology: Preface (2007) Advances in Experimental Medicine and Biology, 616, pp. xii-xiii.
León, R., Fernández, E. Nuclear transformation of eukaryotic microalgae: Historical overview, achievements and problems (2007) Advances in Experimental Medicine and Biology, 616, pp. 1-11. DOI: 10.1007/978-0-387-75532-8_1
Galván, A., González-Ballester, D., Fernández, E. Insertional mutagenesis as a tool to study genes/functions in Chlamydomonas (2007) Advances in Experimental Medicine and Biology, 616, pp. 77-89. DOI: 10.1007/978-0-387-75532-8_7
Merchant, S.S., Prochnik, S.E., Vallon, O., Harris, E.H., Karpowicz, S.J., Witman, G.B., Terry, A., Salamov, A., Fritz-Laylin, L.K., Maréchal-Drouard, L., Marshall, W.F., Qu, L.-H., Nelson, D.R., Sanderfoot, A.A., Spalding, M.H., Kapitonov, V.V., Ren, Q., Ferris, P., Lindquist, E., Shapiro, H., Lucas, S.M., Grimwood, J., Schmutz, J., Grigoriev, I.V., Rokhsar, D.S., Grossman, A.R., Cardol, P., Cerutti, H., Chanfreau, G., Chen, C.-L., Cognat, V., Croft, M.T., Dent, R., Dutcher, S., Fernández, E., Fukuzawa, H., González-Ballester, D., González-Halphen, D., Hallmann, A., Hanikenne, M., Hippler, M., Inwood, W., Jabbari, K., Kalanon, M., Kuras, R., Lefebvre, P.A., Lemaire, S.D., Lobanov, A.V., Lohr, M., Manuell, A., Meier, I., Mets, L., Mittag, M., Mittelmeier, T., Moroney, J.V., Moseley, J., Napoli, C., Nedelcu, A.M., Niyogi, K., Novoselov, S.V., Paulsen, I.T., Pazour, G., Purton, S., Ral, J.-P., Riaño-Pachón, D.M., Riekhof, W., Rymarquis, L., Schroda, M., Stern, D., Umen, J., Willows, R., Wilson, N., Zimmer, S.L., Allmer, J., Balk, J., Bisova, K., Chen, C.-J., Elias, M., Gendler, K., Hauser, C., Lamb, M.R., Ledford, H., Long, J.C., Minagawa, J., Page, M.D., Pan, J., Pootakham, W., Roje, S., Rose, A., Stahlberg, E., Terauchi, A.M., Yang, P., Ball, S., Bowler, C., Dieckmann, C.L., Gladyshev, V.N., Green, P., Jorgensen, R., Mayfield, S., Mueller-Roeber, B., Rajamani, S., Sayre, R.T., Brokstein, P., Dubchak, I., Goodstein, D., Hornick, L., Huang, Y.W., Jhaveri, J., Luo, Y., Martínez, D., Ngau, W.C.A., Otillar, B., Poliakov, A., Porter, A., Szajkowski, L., Werner, G., Zhou, K. The Chlamydomonas genome reveals the evolution of key animal and plant functions (2007) Science, 318 (5848), pp. 245-251. DOI: 10.1126/science.1143609
Ermilova, E.V., Nikitin, M.M., Fernández, E. Chemotaxis to ammonium/methylammonium in Chlamydomonas reinhardtii: The role of transport systems for ammonium/methylammonium (2007) Planta, 226 (5), pp. 1323-1332. DOI: 10.1007/s00425-007-0568-1
Fernández, E., Galván, A. Inorganic nitrogen assimilation in Chlamydomonas (2007) Journal of Experimental Botany, 58 (9), pp. 2279-2287. DOI: 10.1093/jxb/erm106
León, R., Couso, I., Fernández, E. Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii (2007) Journal of Biotechnology, 130 (2), pp. 143-152. DOI: 10.1016/j.jbiotec.2007.03.005
Llamas, Á., Tejada-Jimenez, M., González-Ballester, D., Higuera, J.J., Schwarz, G., Galván, A., Fernández, E. Chlamydomonas reinhardtii CNX1E reconstitutes molybdenum cofactor biosynthesis in Escherichia coli mutants (2007) Eukaryotic Cell, 6 (6), pp. 1063-1067. DOI: 10.1128/EC.00072-07
Camargo, A., Llamas, Á., Schnell, R.A., Higuera, J.J., González-Ballester, D., Lefebvre, P.A., Fernández, E., Galván, A. Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas (2007) Plant Cell, 19 (11), pp. 3491-3503. DOI: 10.1105/tpc.106.045922
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Mariscal, V., Moulin, P., Orsel, M., Miller, A.J., Fernández, E., Galván, A. Differential Regulation of the Chlamydomonas Nar1 Gene Family by Carbon and Nitrogen (2006) Protist, 157 (4), pp. 421-433. DOI: 10.1016/j.protis.2006.06.003
Fischer, K., Llamas, A., Tejada-Jimenez, M., Schrader, N., Kuper, J., Ataya, F.S., Galván, A., Mendel, R.R., Fernández, E., Schwarz, G. Function and structure of the molybdenum cofactor carrier protein from Chlamydomonas reinhardtii (2006) Journal of Biological Chemistry, 281 (40), pp. 30186-30194. DOI: 10.1074/jbc.M603919200
González-Ballester, D., de Montaigu, A., Galván, A., Fernández, E. Corrigendum to "Restriction enzyme site-directed amplification PCR: A tool to identify regions flanking a marker DNA" (2006) Analytical Biochemistry, 353 (2), p. 302. DOI: 10.1016/j.ab.2005.12.021
Galván, A., Mariscal, V., González-Ballester, D., Fernández, E. The green alga Chlamydomonas as a tool to study the nitrate assimilation pathway in plants (2006) Model Plants and Crop Improvement, pp. 125-158. DOI: 10.1201/9780849330636
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Pérez-Alegre, M., Dubus, A., Fernández, E. REM1, a new type of long terminal repeat retrotransposon in Chlamydomonas reinhardtii (2005) Molecular and Cellular Biology, 25 (23), pp. 10628-10638. DOI: 10.1128/MCB.25.23.10628-10638.2005
González-Ballester, D., De Montaigu, A., Galván, A., Fernández, E. Restriction enzyme site-directed amplification PCR: A tool to identify regions flanking a marker DNA (2005) Analytical Biochemistry, 340 (2), pp. 330-335. DOI: 10.1016/j.ab.2005.01.031
Navarro, M.T., Mariscal, V., Macías, M.I., Fernández, E., Galván, A. Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: Isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation (2005) Photosynthesis Research, 83 (2), pp. 151-161. DOI: 10.1007/s11120-004-9297-5
González-Ballester, D., De Montaigu, A., Higuera, J.J., Galván, A., Fernández, E. Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas (2005) Plant Physiology, 137 (2), pp. 522-533. DOI: 10.1104/pp.104.050914
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González-Ballester, D., Camargo, A., Fernández, E. Ammonium transporter genes in Chlamydomonas: The nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression (2004) Plant Molecular Biology, 56 (6), pp. 863-878. DOI: 10.1007/s11103-004-5292-7
Mariscal, V., Rexach, J., Fernández, E., Galván, A. The plastidic nitrite transporter NAR1;1 improves nitrate use efficiency for growth in Clamydomonas (2004) Plant, Cell and Environment, 27 (10), pp. 1321-1328. DOI: 10.1111/j.1365-3040.2004.01239.x
León-Bañares, R., González-Ballester, D., Galván, A., Fernández, E. Transgenic microalgae as green cell-factories (2004) Trends in Biotechnology, 22 (1), pp. 45-52. DOI: 10.1016/j.tibtech.2003.11.003
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Ataya, F.S., Witte, C.P., Galván, A., Igeño, M.I., Fernández, E. Mcp1 encodes the molybdenum cofactor carrier protein in Chlamydomonas reinhardtii and participates in protection, binding, and storage functions of the cofactor (2003) Journal of Biological Chemistry, 278 (13), pp. 10885-10890. DOI: 10.1074/jbc.M211320200
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Rexach, J., Llamas, A., Fernández, E., Galván, A. The activity of the high-affinity nitrate transport system I (NRT2;1, NAR2) is responsible for the efficient signalling of nitrate assimilation genes in Chlamydomonas reinhardtii (2002) Planta, 215 (4), pp. 606-611. DOI: 10.1007/s00425-002-0778-5
Llamas, A., Igeño, M.I., Galván, A., Fernández, E. Nitrate signalling on the nitrate reductase gene promoter depends directly on the activity of the nitrate transport systems in Chlamydomonas (2002) Plant Journal, 30 (3), pp. 261-271. DOI: 10.1046/j.1365-313X.2002.01281.x
Thyssen, C., Van Hunnik, E., Navarro, M.T., Fernández, E., Galván, A., Sültemeyer, D. Analysis of Chlamydomonas mutants with abnormal expression of CO2 and HCO3- uptake systems (2002) Functional Plant Biology, 29 (2-3), pp. 251-260. DOI: 10.1071/pp01198
Galván, A., Rexach, J., Mariscal, V., Fernández, E. Nitrite transport to the chloroplast in Chlamydomonas reinhardtii: Molecular evidence for a regulated process (2002) Journal of Experimental Botany, 53 (370), pp. 845-853. DOI: 10.1093/jexbot/53.370.845
Gómez, I., Merchán, F., Fernández, E., Quesada, A. NADP-malate dehydrogenase from Chlamydomonas: Prediction of new structural determinants for redox regulation by homology modelling (2002) Plant Molecular Biology, 48 (3), pp. 211-221. DOI: 10.1023/A:1013338407266
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Pozuelo, M., MacKintosh, C., Galván, A., Fernández, E. Cytosolic glutamine synthetase and not nitrate reductase from the green alga Chlamydomonas reinhardtii is phosphorylated and binds 14-3-3 proteins (2001) Planta, 212 (2), pp. 264-269. DOI: 10.1007/s004250000388
Galván, A., Fernández, E. Eukaryotic nitrate and nitrite transporters (2001) Cellular and Molecular Life Sciences, 58 (2), pp. 225-233. DOI: 10.1007/PL00000850
Merchán, F., Van den Ende, H., Fernández, E., Beck, C.F. Low-expression genes induced by nitrogen starvation and subsequent sexual differentiation in Chlamydomonas reinhardtii, isolated by the differential display technique (2001) Planta, 213 (2), pp. 309-317. DOI: 10.1007/s004250100567
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Llamas, A., Kalakoutskii, K.L., Fernández, E. Molybdenum cofactor amounts in Chlamydomonas reinhardtii depend on the Nit5 gene function related to molybdate transport (2000) Plant, Cell and Environment, 23 (11), pp. 1247-1255. DOI: 10.1046/j.1365-3040.2000.00631.x
Zhou, J.-J., Fernández, E., Galván, A., Miller, A.J. Erratum: A high-affinity nitrate transport system from Chlamydomonas requires two gene products (FEBS 23233) (FEBS Letters 466 (2000) (225-227) PII: S0014579300010851)) (2000) FEBS Letters, 481 (1), p. 88. DOI: 10.1016/S0014-5793(00)01955-4
Zhou, J.-J., Fernández, E., Galván, A., Miller, A.J. A high-affinity nitrate transport system from Chlamydomonas requires two gene products (2000) FEBS Letters, 466 (2-3), pp. 225-227. DOI: 10.1016/S0014-5793(00)01085-1
Pozuelo, M., Merchán, F., Macías, M.I., Beck, C.F., Galván, A., Fernández, E. The negative effect of nitrate on gametogenesis is independent of nitrate assimilation in Chlamydomonas reinhardtii (2000) Planta, 211 (2), pp. 287-292. DOI: 10.1007/s004250000291
Rexach, J., Fernández, E., Galván, A. The Chlamydomonas reinhardtii Nar1 gene encodes a chloroplast membrane protein involved in nitrite transport (2000) Plant Cell, 12 (8), pp. 1441-1453. DOI: 10.1105/tpc.12.8.1441
Quesada, A., Gómez-García, Ia., Fernández, E. Involvement of chloroplast and mitochondria redox valves in nitrate assimilation
(2000) Trends in Plant Science, 5 (11), pp. 463-464. DOI: 10.1016/S1360-1385(00)01770-2
Navarro, M.T., Guerra, E., Fernández, E., Galván, A. Nitrite reductase mutants as an approach to understanding nitrate assimilation in Chlamydomonas reinhardtii (2000) Plant Physiology, 122 (1), pp. 283-289. DOI: 10.1104/pp.122.1.283
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Rexach, J., Montero, B., Fernández, E., Galván, A. Differential regulation of the high affinity nitrite transport systems III and IV in Chlamydomonas reinhardtii (1999) Journal of Biological Chemistry, 274 (39), pp. 27801-27806. DOI: 10.1074/jbc.274.39.27801
Quiñones, M.A., Galván, A., Fernández, E., Aparicio, P.J. Blue-light requirement for the biosynthesis of an NO2- transport system in the Chlamydomonas reinhardtii nitrate transport mutant S10 (1999) Plant, Cell and Environment, 22 (9), pp. 1169-1175. DOI: 10.1046/j.1365-3040.1999.00480.x
Quesada, A., Gómez, I., Fernández, E. Clustering of the nitrite reductase gene and a light-regulated gene with nitrate assimilation loci in Chlamydomonas reinhardtii (1998) Planta, 206 (2), pp. 259-265. DOI: 10.1007/s004250050398
Witte, C.-P., Igeño, M.I., Mendel, R., Schwarz, G., Fernández, E. The Chlamydomonas reinhardtii MoCo carrier protein is multimeric and stabilizes molybdopteria cofactor in a molybdate charged form (1998) FEBS Letters, 431 (2), pp. 205-209. DOI: 10.1016/S0014-5793(98)00756-X
Quesada, A., Hidalgo, J., Fernández, E. Three Nrt2 genes are differentially regulated in Chlamydomonas reinhardtii (1998) Molecular and General Genetics, 258 (4), pp. 373-377. DOI: 10.1007/s004380050743
Quesada, A., Hidalgo, J., Fernández, E. Corrigendum: Three Nrt2 genes are differentially regulated in Chlamydomonas reinhardtii. Molecular and General Genetics (1998) 258.373-377. DOI: 10.1007/s004380050848
Quesada, A., Krapp, A., Trueman, L.J., Daniel-Vedele, F., Fernández, E., Forde, B.G., Caboche, M. PCR-identification of a Nicotiana plumbaginifolia cDNA homologous to the high-affinity nitrate transporters of the crnA family (1997) Plant Molecular Biology, 34 (2), pp. 265-274. DOI: 10.1023/A:1005872816881
Kalakoutskii, K.L., Fernández, E. Different forms of molybdenum cofactor in Vicia faba seeds: The presence of molybdenum cofactor carrier protein and its purification (1997) Planta, 201 (1), pp. 64-70. DOI: 10.1007/BF01258681
Galván, A., Quesada, A., Fernández, E. The use of mutants to study nitrate assimilation, in green microalgae (1996) Scientia Marina, 60 (SUPPL. 1), pp. 191-194.
Prieto, R., Dubus, A., Galván, A., Fernández, E. Isolation and characterization of two new negative regulatory mutants for nitrate assimilation in Chlamydomonas reinhardtii obtained by insertional mutagenesis (1996) Molecular and General Genetics, 251 (4), pp. 461-471. DOI: 10.1007/s004380050190
Galván, A., Quesada, A., Fernández, E. Nitrate and nitrite are transported by different specific transport systems and by a bispecific transporter in Chlamydomonas reinhardtii (1996) Journal of Biological Chemistry, 271 (4), pp. 2088-2092. DOI: 10.1074/jbc.271.4.2088
Navarro, M.T., Prieto, R., Fernández, E., Galván, A. Constitutive expression of nitrate reductase changes the regulation of nitrate and nitrite transporters in Chlamydomonas reinhardtii (1996) Plant Journal, 9 (6), pp. 819-827. DOI: 10.1046/j.1365-313X.1996.9060819.x
Merchán, F., Kindle, K.L., Llama, M.J., Serra, J.L., Fernández, E. Cloning and sequencing of the nitrate transport system from the thermophilic, filamentous cyanobacterium Phormidium laminosum: comparative analysis with the homologous system from Synechococcus sp. PCC 7942 (1995) Plant Molecular Biology, 28 (4), pp. 759-766. DOI: 10.1007/BF00021199
Merchán, F., Prieto, R., Kindle, K.L., Llama, M.J., Serra, J.L., Fernández, E. Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum (1995) Plant Molecular Biology, 27 (5), pp. 1037-1042. DOI: 10.1007/BF00037030
Kalakoutskii, K.L., Fernández, E. Chlamydomonas reinhardtii nitrate reductase complex has 105 kDa subunits in the wild-type strain and a structural mutant (1995) Plant Science, 105 (2), pp. 195-206. DOI: 10.1016/0168-9452(94)04052-4
Caboche, M., Campbell, W., Crawford, N.M., Fernández, E., Kleinhofs, A., Ida, S., Mendel, R., Omata, T., Rothstein, S., Wray, J. Genes involved in nitrate assimilation (1994) Plant Molecular Biology Reporter, 12 (2), pp. S45-S49. DOI: 10.1007/BF02671569
Quesada, A., Galván, A., Fernández, E. Identification of nitrate transporter genes in Chlamydomonas reinhardtii (1994) The Plant Journal, 5 (3), pp. 407-419. DOI: 10.1111/j.1365-313X.1994.00407.x
Quesada, A., Fernández, E. Expression of nitrate assimilation related genes in Chlamydomonas reinhardtii (1994) Plant Molecular Biology, 24 (1), pp. 185-194. DOI: 10.1007/BF00040584
Quesada, A., Galván, A., Schnell, R.A., Lefebvre, P.A., Fernández, E. Five nitrate assimilation-related loci are clustered in Chlamydomonas reinhardtii (1993) MGG Molecular & General Genetics, 240 (3), pp. 387-394. DOI: 10.1007/BF00280390
Prieto, R., Fernández, E. Toxicity of and mutagenesis by chlorate are independent of nitrate reductase activity in Chlamydomonas reinhardtii (1993) MGG Molecular & General Genetics, 237 (3), pp. 429-438. DOI: 10.1007/BF00279448
Aguilar, M., Cárdenas, J., Fernández, E. Quantitation of molybdopterin oxidation product in wild-type and molybdenum cofactor deficient mutants of Chalamydomonas reinhardtii (1992) Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 1160 (3), pp. 269-274. DOI: 10.1016/0167-4838(92)90087-T
Aguilar, M., Kalakoutskii, K., Cárdenas, J., Fernández, E. Direct transfer of molybdopterin cofactor to aponitrate reductase from a carrier protein in Chlamydomonas reinhardtii (1992) FEBS Letters, 307 (2), pp. 162-163. DOI: 10.1016/0014-5793(92)80758-9
Galván, A., Cárdenas, J., Fernández, E. Nitrate reductase regulates expression of nitrite uptake and nitrite reductase activities in Chlamydomonas reinhardtii (1992) Plant Physiology, 98 (2), pp. 422-426. DOI: 10.1104/pp.98.2.422
Aguilar, M.R., Prieto, R., Cárdenas, J., Fernández, E. Nit 7: A new locus for molybdopterin cofactor biosynthesis in the green alga Chlamydomonas reinhardtii (1992) Plant Physiology, 98 (1), pp. 395-398. DOI: 10.1104/pp.98.1.395
Galván, A., Córdoba, F., Cárdenas, J., Fernández, E. Regulation of nitrite uptake and nitrite reductase expression in Chlamydomonas reinhardtii (1991) BBA - General Subjects, 1074 (1), pp. 6-11. DOI: 10.1016/0304-4165(91)90030-K
Aguilar, M.R., Cárdenas, J., Fernández, E. Regulation of molybdenum cofactor species in the green alga Chlamydomonas reinhardtii (1991) BBA - General Subjects, 1073 (3), pp. 463-469. DOI: 10.1016/0304-4165(91)90216-4
Kindle, K.L., Schnell, R.A., Fernández, E., Lefebvre, P.A. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase (1989) Journal of Cell Biology, 109 (6 I), pp. 2589-2601. DOI: 10.1083/jcb.109.6.2589
Fernández, E., Schnell, R., Ranum, L.P., Hussey, S.C., Silflow, C.D., Lefebvre, P.A. Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. (1989) Proceedings of the National Academy of Sciences of the United States of America, 86 (17), pp. 6449-6453. DOI: 10.1073/pnas.86.17.6449
Franco, A.R., Cárdenas, J., Fernández, E. Regulation by ammonium of nitrate and nitrite assimilation in Chlamydomonas reinhardtii (1988) BBA - Gene Structure and Expression, 951 (1), pp. 98-103. DOI: 10.1016/0167-4781(88)90029-2
Franco, A.R., Cárdenas, J., Fernández, E. Two different carriers transport both ammonium and methylammonium in Chlamydomonas reinhardtii. (1988) The Journal of biological chemistry, 263 (28), pp. 14039-14043.
Franco, A.R., Cárdenas, J., Fernández, E. A mutant of Chlamydomonas reinhardtii altered in the transfort of ammonium and methylammonium (1987) MGG Molecular & General Genetics, 209 (3), p. 633. DOI: 10.1007/BF00331177
Córdoba, F., Cárdenas, J., Fernández, E. Cooperative regulation by ammonium and ammonium derivatives of nitrite uptake in Chlamydomonas reinhardtii (1987) BBA - Biomembranes, 902 (3), pp. 287-292. DOI: 10.1016/0005-2736(87)90196-9
Fernández, E., Aguilar, M. Molybdate repair of molybdopterin deficient mutants from Chlamydomonas reinhardtii (1987) Current Genetics, 12 (5), pp. 349-355. DOI: 10.1007/BF00405757
Franco, A.R., Cárdenas, J., Fernández, E. A mutant of Chlamydomonas reinhardtii altered in the transport of ammonium and methylammonium (1987) MGG Molecular & General Genetics, 206 (3), pp. 414-418. DOI: 10.1007/BF00428880
Fernández, E., Matagne, R.F. In vivo complementation analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardtii (1986) Current Genetics, 10 (5), pp. 397-403. DOI: 10.1007/BF00418413
Galván, F., Márquez, A.J., Fernández, E. Physicochemical Properties of Ferredoxin from Chlamydomonas reinhardii (1985) Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 40 (5-6), pp. 373-378. DOI: 10.1515/znc-1985-5-615
Córdoba, F., Cárdenas, J., Fernández, E. Role of the diaphorase moiety on the reversible inactivation of the Chlamydomonas reinhardii nitrate reductase complex (1985) Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 827 (1), pp. 8-13. DOI: 10.1016/0167-4838(85)90094-9
Franco, A.R., Cárdenas, J., Fernández, E. Ammonium (methylammonium) is the co-repressor of nitrate reductase in Chlamydomonas reinhardii (1984) FEBS Letters, 176 (2), pp. 453-456. DOI: 10.1016/0014-5793(84)81217-X
Fernández, E., Matagne, R.F. Genetic analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardii (1984) Current Genetics, 8 (8), pp. 635-640. DOI: 10.1007/BF00395710
Pineda, M., Fernández, E., Cárdenas, J. Urate oxidase of Chlamydomonas reinhardii (1984) Physiologia Plantarum, 62 (3), pp. 453-457. DOI: 10.1111/j.1399-3054.1984.tb04602.x
Fernández, E. Nitrate Reductase from a Mutant Strain of Chlamydomonas reinhardii Incapable of Nitrate Assimilation (1983) Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 38 (5-6), pp. 439-445. DOI: 10.1515/znc-1983-5-618
Fernández, E., Cardenas, J. Isolation and properties of the NAD(P)H-cytochrome c reductase subunit of Chlamydomonas reinhardii NAD(P)H-nitrate reductase (1983) Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 745 (1), pp. 12-19. DOI: 10.1016/0167-4838(83)90164-4
Fernández, E., Cárdenas, J. Isoelectric focusing of the NAD(P)H-cytochrome c reductase subunit of Chlamydomonas reinhardii nitrate reductase (1983) Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 38 (1-2), pp. 35-38. DOI: 10.1515/znc-1983-1-208
Fernández, E., Cardenas, J. Biochemical characterization of a singular mutant of nitrate reductase from Chlamydomonas reinhardii. New evidence for a heteropolymeric enzyme structure (1982) BBA - Bioenergetics, 681 (3), pp. 530-537. DOI: 10.1016/0005-2728(82)90196-7
Fernández, E., Cárdenas, J. Regulation of the nitrate-reducing system enzymes in wild-type and mutant strains of Chlamydomonas reinhardii (1982) MGG Molecular & General Genetics, 186 (2), pp. 164-169. DOI: 10.1007/BF00331846
Fernández, E., Cárdenas, J. Occurrence of xanthine dehydrogenase in Chlamydomonas reinhardii: A common cofactor shared by xanthine dehydrogenase and nitrate reductase (1981) Planta, 153 (3), pp. 254-257. DOI: 10.1007/BF00383895
Fernández, E., Cárdenas, J. In vitro complementation of assimilatory NAD(P)H-nitrate reductase from mutants of Chlamydomonas reinhardii (1981) BBA - Enzymology, 657 (1), pp. 1-12. DOI: 10.1016/0005-2744(81)90125-X